Akbar Yaghobfar; Rezvan Yaghoubfar; Ehsan Zare Banadkoki
Volume 23, Issue 2 , July 2021, , Pages 223-233
Abstract
The experiment was conducted to investigate the effects of cell wall carbohydrates with diet supplemented enzyme on the function and expression of glucose transporter genes (SGLT1 and GLUT2), peptide transporter (PepT1) and mucin production (MUC2) in the small intestine of broilers. In this study, 1100 ...
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The experiment was conducted to investigate the effects of cell wall carbohydrates with diet supplemented enzyme on the function and expression of glucose transporter genes (SGLT1 and GLUT2), peptide transporter (PepT1) and mucin production (MUC2) in the small intestine of broilers. In this study, 1100 mixeddayold chickens (male and female), Ross 308 were used based on a completely randomized design with 11 treatments and five replications (20 birds per replication) for 42 days. Experimental diets included control diets, diets containing wheat, barley, wheat bran, rice bran, and hull less barley with and without enzymes, respectively. The results showed that the effect of diets containing wheat, barley and hull less barley with enzyme on the total live weight of broiler chickens at 42 days of age was significantly different (P <0.05).Cell wall carbohydrates of wheat, wheat bran and rice bran in diets increased pancreatic amylase activity (barley 3.02, wheat 5.99 U/mg CP of small intestinal tissue) (P < 0.05). The expression of the studied SGLT1 and MUC2 genes in the experimental diets without enzyme showed a significant increase compared to enzymes supplemented diet (P < 0.05). Also, among the groups of enzyme-supplemented diets, only wheat and rice bran groups were able to increase the expression of SGLT1, MUC2 and GLUT2 genes compared to the control group (P <0.05). In conclusion, supplementation of diets containing cell wall carbohydrates with enzyme affects the expression of glucose transport genes (SGLT1 and GLUT2), peptide transport (PepT1) and mucin production (MUC2) in the small intestine jejunum. This indicates the optimal function of the digestive system of broilers in terms of digestion and absorption of nutrients.
Drakhshanda Rahimhi; i Hossein Mansoori Yarahmad; Akbar Yaghobfar; Jafar Fakhraei
Volume 22, Issue 1 , March 2020, , Pages 117-128
Abstract
For carry out of this study we used 560 Ross 308 (mixed sex) broiler chickens, in a completely randomized design with seven t reatments, four replicates and 20 chickens per each replicate. Experimental treatments were: control (no additive), diets containing levels of 400 and 500 mg/kg Satureja ...
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For carry out of this study we used 560 Ross 308 (mixed sex) broiler chickens, in a completely randomized design with seven t reatments, four replicates and 20 chickens per each replicate. Experimental treatments were: control (no additive), diets containing levels of 400 and 500 mg/kg Satureja essential oil, diets containing levels of two and four percent garlic powder and diets containing levels of five and 10 percent clover leaf powder. The results of this study showed that weight gain of birds that fed with diets containing Satureja khuzistanica essential oil, garlic and clover leaf powder was less than the control birds (P<0/05) across the trial period. Experimental treatments had no significant effect on serum concentration of thyroid hormones (T3 and T4), cholesterol, triglyceride, HDL and LDL and percentage of dry matter and crude protein of breast muscle. Also, according to different holding times of breast muscle in the freezer (0, 24 and 48 hours), experimental treatments had no significant effect on the amount of MDA and free N at 24 and 48 hours after freezing. According to the results, using of Satureja essential oil, garlic and clover leaf powder at levels investigated in this study, do not have a significant effect on the performance and the antioxidant potential of breast muscle to modulate lipid per oxidation. Therefore, further studies are needed to evaluate the effectivness and active constituents of these nutrients and ensure their properties.
Fahima Daneshyar; S. Mohammad Hosseini; Akbar Yaghobfar
Volume 21, Issue 2 , July 2019, , Pages 233-246
Abstract
In order to determine the effect of non-starch polysaccarides on performance, energy and protein efficiencies and carcass characteristics of broiler chicks, an experiment was conducted in three periods of starter, grower and finisher period, using 420 one-day old Ross 308 broiler chicks from both sexes ...
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In order to determine the effect of non-starch polysaccarides on performance, energy and protein efficiencies and carcass characteristics of broiler chicks, an experiment was conducted in three periods of starter, grower and finisher period, using 420 one-day old Ross 308 broiler chicks from both sexes in a completely randomized design with seven treatments, six replicates and 10 chicks per replicate. The experimental treatments were basal diet, diets containing 0.10 and 0.20 percent of Saccharomyces cerevisiae yeast, diets containing 5 and 10 percent of wheat bran, diets containing three and six percent of soybean hull. The results of the experiment showed that the control treatment had the lowest feed intake during the whole period of breeding (P<0.05). The highest feed intake was related to 10 percent wheat bran and levels of 3 and 6 percent soybean hull (P<0.05). Treatments containing 3 percent soybean hull showed the highest daily weight gain (P<0.05) during the whole experiment. There is no statistical difference for the feed conversion ratio during the whole trial period. The highest relative energy efficiency related to yeast levels were compared to treatments containing wheat bran and soybean hull (P<0.05). The lowest relative energy and protein efficiency was related to 10 percent wheat bran compared to control treatment (P <0.05). The results of the experiment showed that the use of two tenths of yeast, 10 percent wheat bran and six percent soybean hull in the diet of broiler chickens is recommended.
EMRAN AZARBAD; Hassan kermansh; Akbar Yaghobfar; Amir Meimandipour
Volume 21, Issue 1 , April 2019, , Pages 87-97
Abstract
In order to determine the effects of adding different levels of Satureja khuzistanica essential oilin two conventional and microcapsulated forms into the diet on performance, immune response, blood biochemical parameters and intestinal morphology of broiler chickens, an exprement was conducted using ...
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In order to determine the effects of adding different levels of Satureja khuzistanica essential oilin two conventional and microcapsulated forms into the diet on performance, immune response, blood biochemical parameters and intestinal morphology of broiler chickens, an exprement was conducted using 400 Ross 308 (mixed sex) broiler chickens, in five treatments, four replicates and 20 chickens per each replicate. The expriment was done in a completely randomized design. Treatments included control (no additive), dietary containing levels of 400 and 500 mg/kg Satureja essential oil and dietary containing levels of 0.5 and 1.00 percent capsulated Satureja essential oil. Adding different levels of Satureja khuzistanica essential oil in conventional and microcapsulated forms to diet of broiler chickens had no effect on feed intake,FCR, immune response and the number of white and red blood cells. However, experimental treatments containing conventional and microcapsulatedformof Satureja essential oil caused a significant decrease in body weight of the birds relative to than the control treatment. The level of thyroid hormones (T3 and T4) and blood biochemical parameters (cholesterol, triglyceride, HDL and LDL) were not affected by the experimental treatments. Although small intestin histologhy traits like width of villi, depth of crypt and length of villi to depth of crypt ratio were not affected by the experimental treatments, the level of 0.5 percent capsulated Satureja essential oil caused a significant decrease in length to width of villi ratio (P<0/05). Acccording to the results of the present experiment, using of two common and microcapsulated Satureja essential oil forms, have not any effect on performance, blood biochemical parameters and immune response.
Vajiheh Nikoofard; Akbar Yaghobfar; Shahab Ghazi Harsini; Ali Asghar Saki
Volume 20, Issue 3 , November 2018, , Pages 463-476
Abstract
The effect of diet with different protein quality andsupplemented with crystalline amino acids on performance, immune response, enzyme activity in the intestinal tissue and litter characteristics, using 576 Ross 308 (mixed sex) broiler chickens,in a 2 × 4 factorial experiment with two experimental ...
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The effect of diet with different protein quality andsupplemented with crystalline amino acids on performance, immune response, enzyme activity in the intestinal tissue and litter characteristics, using 576 Ross 308 (mixed sex) broiler chickens,in a 2 × 4 factorial experiment with two experimental diets (high and low protein quality) and four levels of crystalline amino acids (recommended levels, without supplemented crystalline amino acids, 10 and 15% higher than the recommended levels)in a completely randomized design with eight treatments, six replicates and 12 chicks per replicate was investigated.The results of this study showed that, adding crystalline amino acids at levels of 10 and 15% higher than the recommended level to diets containing canola meal and wheat (as low quality protein source) caused an increase in performance (P<0.0001), the number of heterophile and heterophile to lymphocyte ratio (P<0.001) and decreased the number of lymphocyte (P<0.01). The treatments with crystalline amino acids hadn’t significant effect on the enzyme activity aminopeptidase and specific activity of the enzyme in jejunum. Also, adding crystalline amino acids at levels of 10 and 15% higher than the recommended level ina low-quality diet were not significantly affected dry matter, nitrogen and ammonia nitrogen in the litter. According to these results, the use ofcrystalline amino acids higher than the recommended levels in the diets with high quality (as maize-soybean meal) has no effect on the performance of broiler chickens and is not recommended.
mohammad Bojarpour; Akbar Yaghoobfar; somayeh salari
Volume 19, Issue 1 , May 2017, , Pages 159-174
Abstract
This experiment was conducted to evaluate the effects of physical form, barley variety and enzyme addition on Ileal digestibility, apparent metabolizable energy (AME) and energy efficiency of broiler chickens.The experiment was carried using a factorial experiment with a completely randomized desiegn. ...
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This experiment was conducted to evaluate the effects of physical form, barley variety and enzyme addition on Ileal digestibility, apparent metabolizable energy (AME) and energy efficiency of broiler chickens.The experiment was carried using a factorial experiment with a completely randomized desiegn. Factors were barley varity (Fajr, Reyhaneh, and Yosef), diet form (mash or pellet) and enzyme level (0 and 0/05 g/kg dry matter). Here 927 one day-old Ross 308 broiler chickens were assigned to 12 treatments, 3 replicates and 27 chickens in each replicate. The results of experiment indicated that the digestibility of nutrients, AME, apparent metabolizable energy corrected to zero nitrogen retention (AMEn) and energy efficiencies (NE/AMEn) affected by barley variety (p <0/05). Net Energy (NE) affected by main and interaction effects (P < 005). Diets containing Ryhaneh barley variety in comparison with other varietys, increased Ileal Digestibel Protein (IDP), Ileal Digestibel fat (IDF), NE, AME and AMEn (P <0/05). Although pelleting decreased Ileal Digestibility of nutrients (p>005), NE, AME and AMEn (p <0/05), addition of enzyme to diet increased this indicators (P <0/050). According to these results, the use of pellet diet containing Ryhaneh variety with enzyme improved digestibility and energy efficiency and thus it's recommended to be used in poultry nutrition.
Ali Ghodrat; Akbar Yaghobfar; Yahya Ebrahimnezhad; Habib Aghdam Shahryar; Abolfazl Ghorbani
Volume 17, Issue 1 , April 2015, , Pages 39-49
Abstract
This study was carried out to determination of in vitro binding capacity of organic (wheat and barley) and inorganic (perlite) sources for Mn, Zn, Cu, and Fe. For this reason wheat and barley were analyzed chemically for moisture, protein, ash, and ether extract, total carbohydrate, NDF, ADF, ADL, crude ...
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This study was carried out to determination of in vitro binding capacity of organic (wheat and barley) and inorganic (perlite) sources for Mn, Zn, Cu, and Fe. For this reason wheat and barley were analyzed chemically for moisture, protein, ash, and ether extract, total carbohydrate, NDF, ADF, ADL, crude fiber, viscosity, cellulose, and total dietary fiber. The in vitro mineral binding capacity of wheat, barley, and perlite to Mn, Zn, Fe, and Cu under sequential simulated physiological conditions of the stomach, small intestine, and colon was investigated and compared. Acid washing was efficient in removing most endogenous minerals from samples with the exception of Fe. Barley showed the highest mineral binding capacity for Mn (5.16 percent), Zn (11.07 percent), and Cu (44.48 percent) in small intestine. Perlite had lower mineral binding capacity (Mn (0.76 percent), Zn (1.74 percent) and Cu (1.41 percent) than wheat and barley. Organic sources had an affinity for Fe > Cu > Zn > Mn. Dietary Fiber had a negative impact on mineral bioavailability.